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การประเมินความคงตัวของความต้านทานต่อไวรัสใบด่างเขียวในแตงกวาจากสายพันธุ์การค้ารุ่น S2 และ S3 และกลุ่มที่เข้าสู่ inbred lines ที่ต้านทานต่อเชื้อ CGMMV Thai Agricultural
Premruethai Unruean; Kamol Lertrat; Petcharat Thummabenjapone.
Cucumber green mottle mosaic virus (CGMMV) is a member of Tobamovirus, it causes green mottle mosaic disease in cucurbits with the virus being transmitted through seed. The aims of this research were emphasized on evaluation the stability of CGMMV resistant phenotype in self fertilization generation S2 and S3 of new cucumber lines (10 lines) generated from commercial cucumber varieties and tentatively inbred lines(11 lines) from previously screening program. The results showed that the good candidate cucumber lines from commercial source which resistant (R) to moderate resistant (MR) to CGMMV were No.P10 line 6-1, No.P19 line 2-1 and No.P19 line 5-1. The self generation lines derived from No.P3 x P4 showed stability in R-MR phenotype. For tentatively...
Tipo: PhysicalObject Palavras-chave: Cucumber; Cucurbit virus disease; Plant breeding; Disease resistant; Variety screening; Commercial varieties; Inbred line; Cucumber green mottle mosaic virus; CGMMV; Tobamovirus; Green mottle mosaic disease; แตงกวา; เชื้อไวรัส; โรคไวรัสด่างเขียว; การปรับปรุงพันธุ์พืช; ความต้านทานโรค; การคัดเลือก; สายพันธุ์การค้า; สายพันธุ์แท้; ชั่วรุ่นผสมตัวเอง; การประเมินลักษณะความต้านทาน.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5772
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การผลิตโพลีโคลนอลแอนติซีรัมที่จำเพาะต่อ Streptomyces-PR15 และการใช้ตรวจติดตามเชื้อรอบผิวรากต้นกล้าพริก Thai Agricultural
Weerakorn Saengsai; Petcharat Thummabenjapone.
The antagonistic Streptomyces-PR15 is a broad spectrum against various plant pathogenic fungi and bacteria of economic crops. To develop the ELISA technique for detection the Streptomyces-PR15 in bioproducts and plant surrounding environment, the production of polyclonal antiserum against Streptomyces-PR15 was carried out. The antigen prepared from Streptomyces-PR15 whole cell proteins was mixed with Freund’s incomplete adjuvant (1:1, v/v) was injected to in New Zealand White rabbit with immunization program as follows, intramuscular injection for 3 times then only antigen was intravenous injected for 2 times. Bleeding was done at 10 days after final immunization and then at weekly interval for 8 weeks. The antigen was re-intravenous injected at 14 days...
Tipo: PhysicalObject Palavras-chave: Pepper; Chilli seedlings; Antagonist; Streptomyces-PR15; Polyclonal antiserum; Bioproduct; Elisa; Antiserum against; พริกหนุ่มเขียว; พริกขี้หนู; รากต้นกล้า; จุลินทรีย์ปฏิปักษ์; เชื้อแบคทีเรีย สเตรปโตมัยซีส; โพลีโคลนอลแอนติบอดี; การตรวจติดตามเชื้อ; ชีวภัณฑ์; คุณภาพแอนติซีรัม; แอนติบอดีจำเพาะ.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5804
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ความสัมพันธ์ของปริมาณ Heat Shock Protein 70 (HSP70) กับคุณภาพน้ำเชื้อในพ่อพันธุ์โคนมลูกผสมโฮลสไตน์ฟรีเชี่ยน Thai Agricultural
Nipa Nasinprom; Suporn Katawatin; Monchai Duangjinda; Petcharat Thummabenjapone.
This study attempted to clarify the appearance of 70 kDa heat shock protein (HSP70) in spermatozoa and its relationship with the semen quality in crossbred Holstein Friesian (HF) bulls. Semen from 10 HF bulls was collected 8 weeks during rainy (THI = 79.06), winter (THI = 74.30), and summer (THI = 80.05). Semen quality was evaluated in terms of semen volume, sperm concentration, sperm motility, sperm viability, and percentage of abnormal spermatozoa. It was found that mass movement of sperm in the summer and rainy seasons were significantly lower than in the winter (P<0.01). The percentage of abnormal sperm were significantly lowest in the summer (P<0.01). The western blot analysis of the levels of HSP70 in spermatozoa found that it was significantly...
Tipo: Collection Palavras-chave: Semen quality; Holstein Friesian bulls; HSP70; คุณภาพโคนม; น้ำเชื้อ; โคนม; อสุจิ.
Ano: 2009 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5957
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วิธีการใช้ Streptomyces-PR87 ในการส่งเสริมการเจริญเติบโตของมะเขือเทศ Thai Agricultural
Assiri Klangsawad; Petcharat Thummabenjapone.
Using plant growth promoting microbes is an alternative approach for reducing cost and enhancing good health of crop plants. This research aims to establish the application protocol for enhance growth of tomato at seedling and transplanting stages by antagonistic Streptomyces-PR87 which will be applied further to farmer. The experiments were carried out using two varieties of tomato, e.g. var.Sida and var.Tomato32. In seedling stage, the experiments were carried out in combinations with Streptomyces-PR87 concentration at OD600=0.2, 1 and 5 and drenching interval at 3, 5 or 7 days, and seed soaking for 24 hours before sowing or directly drenching after seed sowing methods. The results showed that the most appropriated method for enhance growth and...
Tipo: PhysicalObject Palavras-chave: Organic tomato; Microorganism; Biofertilizer; Actinomycetes; Growth promote; Bioregulators; Plant disease control; Germination; Streptomyces-PR87; มะเขือเทศ; เชื้อจุลินทรีย์; การส่งเสริมการเจริญเติบโต; ปุ๋ยชีวภาพ; แอคติโนมัยซีส; เชื้อแบคทีเรียปฏิปักษ์; สารควบคุมการเจริญเติบโต; การควบคุมโรค; การงอก; ความยาวของราก; ความสูงของลำต้น; การปลูกพืชอินทรีย์.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5785
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ศักยภาพของเชื้อ Streptomyces-PR87 ปฏิปักษ์ และวิธีการใช้สำหรับควบคุมไส้เดือนฝอยรากปม (Meloidogyne incognita) ในสภาพโรงเรือนปลูกพืชทดลอง Thai Agricultural
Rattikan Yutthasin; Petcharat Thummabenjapone; Anan Hiransalee.
The root-knot nematode (Meloidogyne incognita) caused root knot disease of several economic crops. This research aims to evaluate the potential of antagonistic Streptomyces-PR87 to control root knot nematode under laboratory condition and determines the suitable application to tomato root knot disease on under green house condition. The results showed that the culture filtrate of Streptomyces-PR87 obtained from 7 days old culture in Arginine glycerol mineral salt broth at concentration of 50, 75 and 100% significantly inhibited egg hatching of M. incognita at average of the number of second-stage juvenile (J2) larvae per 5 egg masses with 37.67, 6.67 and 3.33 larvae of J2 per 5 egg masses, respectively compared of 136.33 J2 from 5 egg masses in distilled...
Tipo: PhysicalObject Palavras-chave: Tomato; Tomato nematode; Root-knot nematode; Actinomycetes; Streptomyces-PR87; Microorganism; Biocontrol; Plant disease control; Pest control methods; มะเขือเทศ; ไส้เดือนฝอยรากปม; โรครากปม; แอคติโนมัยซีส; เชื้อจุลินทรีย์; เชื้อแบคทีเรียปฏิปักษ์; การควบคุมศัตรูพืช; การควบคุมโรค; การควบคุมโดยชีววิธี.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5786
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